✦ LIBER ✦
Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions
✍ Scribed by Trapani, Stefano ;Schoehn, Guy ;Navaza, Jorge ;Abergel, Chantal
- Book ID
- 104478535
- Publisher
- International Union of Crystallography
- Year
- 2010
- Tongue
- English
- Weight
- 841 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0907-4449
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✦ Synopsis
The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing negative staining, a technique that is of wider applicability than cryo-electron microscopy, to produce models of medium-size proteins suitable for molecular replacement. The technique was used to solve the crystal structure of the dodecameric type II dehydroquinase enzyme from__Candida albicans__(∼190 kDa) and that of the orthologous__Streptomyces coelicolor__protein.