โ Scribed by Joel D. Richter (Eds.)
No coin nor oath required. For personal study only.
mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed.
Key Features
Isolation and characterization of specific RNA binding proteins
RNA metabolism and associated regulatory proteins
RNA detection and localization
A genetic approach to RNA function
Content:
Contributors, Pages xiii-xv
Preface, Page xvii, Joel D. Richter
Chapter 1 - The TatโTAR RNP, a Master Switch That Regulates HIV-1 Gene Expression, Pages 1-23, Carlos Suรฑรฉ, Yi Liu, Paul R. Bohjanen, Mariano A. Garcia-Blanco
Chapter 2 - HeLa Nuclear Extract: A Modified Protocol, Pages 25-30, Cameron R. Miller, Sharon F. Jamison, Mariano A. Garcia-Blanco
Chapter 3 - Functional Analysis of Splicing Factors and Regulators, Pages 31-53, Juan Valcรกrcel, Concepciรณn Martรญnez, Michael R. Green
Chapter 4 - Identification of pre-mRNA Splicing Factors and Analysis of RNAโProtein Interaction, Pages 55-78, James G. Patton, Daron C. Barnard, Billy T. Dye, James G. McAfee
Chapter 5 - In Vitro Analysis of Mammalian Cell mRNA 3โฒ Processing, Pages 79-98, Gregory M. Gilmartin
Chapter 6 - Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins, Pages 99-109, Jeffrey Wilusz
Chapter 7 - Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae, Pages 111-125, J. Scott Butler, Michael W. Briggs, Aaron Proweller
Chapter 8 - Poly (A) Polymerase/Cap-Specific 2โฒ-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and ProteinโLigand Interaction Assays, Pages 127-148, Paul D. Gershon
Chapter 9 - A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast, Pages 149-161, Aidan N. Hennigan, Allan Jacobson
Chapter 10 - Identification of the Protein That Interacts with the 3โฒ End of Histone mRNA, Pages 163-193, Williams F. Marzluff, Zbigniew Dominski, Michael L. Whitfield, Zeng-Feng Wang
Chapter 11 - Analysis of mRNP Complexes Assembled in Vitro, Pages 195-209, Martin Holcik, Stephen A. Liebhaber
Chapter 12 - Analysis of RNA Binding Proteins Using in Vitro Genetics, Pages 211-236, Ite A. Laird-Offringa
Chapter 13 - Interactions of Proteins with Specific Sequences in RNA, Pages 237-261, Lucy G. Andrews, Jack D. Keene
Chapter 14 - A Rapid Genetic Method for the Study of RNA Binding Proteins, Pages 263-284,I, Chaitanya Jain, Joel G. Belasco
Chapter 15 - Footprinting RNAโProtein Complexes with Hydroxyl Radicals, Pages 285-303, Patricia Ansel-McKinney, Lee Gehrke
Chapter 16 - Analysis of Ribosome Loading onto mRNA Species: Implications for Translational Control, Pages 305-321, Hangjun Ruan, Cheryl Y. Brown, David R. Morris
Chapter 17 - Analysis of Picornavirus Internal Ribosome Entry Site Function in Vivo, Pages 323-340, Graham J. Belsham
Chapter 18 - RNA Traffic and Localization Reported by Fluorescent Molecular Cytochemistry in Living Cells, Pages 341-359, Marty R. Jacobson, Thoru Pederson
Chapter 19 - Detection of mRNA in Situ: Techniques for Studying Gene Expression in Drosophila melanogaster Tissues, Pages 361-370,III, Armen S. Manoukian
Chapter 20 - Differential Display Protocol That Preferentially Identifies mRNAs of Moderate to Low Abundance in a Microscopic System, Pages 371-383, Ognian C. Ikonomov, Michele H. Jacob
Index, Pages 385-395
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