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Lysozyme refolding at high concentration by dilution and size-exclusion chromatography

โœ Scribed by Yong-gui Gao; Yi-xin Guan; Shan-jing Yao; Man-gi Cho

Book ID
111839913
Publisher
SP Zhejiang University Press
Year
2003
Tongue
English
Weight
460 KB
Volume
4
Category
Article
ISSN
1009-3095

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Protein refolding at high concentration
Protein refolding at high concentration using size-exclusion chromatography
โœ Boris Batas; Julian B. Chaudhuri ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 809 KB

A new method to improve refolding yields and t o increase the concentration of refolded proteins in a single operation has been developed. The method uses size-exclusion chromatography matrices to perform buffer exchange, aggregate removal, and the folding reaction. The reduced diffusion of proteins