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Lysine oligopeptides. Preparation by ion-exchange chromatography

โœ Scribed by A. Yaron; M. C. Otey; H. A. Sober; E. Katchalski; S. Ehrlich-Rogozinski; A. Berger

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
727 KB
Volume
11
Category
Article
ISSN
0006-3525

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โœฆ Synopsis

The preparation of L-lysine peptides (Lys,, n = 2-14) from poly-L-lysine is described.

Fractionation by ion-exchange column chromatography of poly-Llysine hydrolysates on apreparative scale resulted in 0.2-1.0g quantities of individual members of the poly-Llysine series. The peptides isolated proved to be analytically pure and the optical configuration was fully retained, as demonstrated by complete enzymic digestion. Peptides higher than n = 14 were also prepared. They cousist,ed of oligolysine groups of narrow and accurately determined size distribution. Potentiometric t.itratioris were used both to characterize the products and to demonstrate t.he charact~eristic dependence of the dissociation constants on size of the peptide.

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