LY-6C+ natural T (NT) cells mediate immune surveillance against NK-sensitive and NK-resistant transplantable tumours in certain strains of mice

✍ Scribed by Rosetta Martiniello; Robert C. Burton; Y. Cheng Smart

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 686 KB
• Volume: 66
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19960516)66:4<532::aid-ijc18>3.0.co;2-9

✦ Synopsis

We have previously shown that anti-Ly-6C monoclonal antibody (MAb) 2B6-F2 identifies a subset of (CBA % C57BL/6)FI splenic NK-I. I + natural T (NT) cells which kill the NK-sensitive YAC-I target in vitro. Furthermore, these Ly-6C+ cells are responsible for 4650% of in vitro YAC-I killing in all mouse strains tested. In BALB/c and DBA/2 mice, these cells killed not only YAC-I but also the NK-resistant WEHI-I64 M I / I 6 target via a receptor that recognises a shared determinant on these targets in vitro. In the present study, the anti-tumour role of Ly-6C+ cells against the NK-sensitive B I 6 melanoma and NKresistant tumours WEHI-7 T lymphoma and WEHI-I64/ I C fibrosarcoma was studied in vitro and in vivo. In vitro, 816, WEHI-7 and WEHI-I64/ I C tumour cell lines were highly sensitive to Ly-6C+ cell killing. In vivo, these same tumours showed significantly increased growth when transplanted S.C. into syngeneic mice treated with 2B6-F2 (0.05 5 p < 0.0005). and this was most marked in the first 15 days following turnour appearance, when tumours were < 15 mm in diameter. Our results show that Ly-6Ct cells play a role in controlling the growth of transplantable NK-sensitive B I6 melanoma, and in BALB/c mice, at least, the repertoire of susceptible tumours is extended to include NK-resistant WEHI-7 and WEHI-I64/ I C. We conclude that Ly-6C+ N T cells play a role in immunosurveillance against NK-sensitive as well as NK-resistant tumours in certain strains of mice.