Lung Innate Immunity and Inflammation

✍ Scribed by Scott Alper, William J. Janssen

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 430
• Series: Methods in Molecular Biology 1809
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.

✦ Table of Contents

Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy (William McKleroy, Kenneth Lyn-Kew)....Pages 3-15
Overview of Innate Lung Immunity and Inflammation (David W. H. Riches, Thomas R. Martin)....Pages 17-30
Front Matter ....Pages 31-31
Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes (Sophie L. Gibbings, Claudia V. Jakubzick)....Pages 33-44
Isolation and Characterization of Mouse Neutrophils (Niki D. J. Ubags, Benjamin T. Suratt)....Pages 45-57
Isolation and Characterization of Mouse Fibroblasts (Benjamin L. Edelman, Elizabeth F. Redente)....Pages 59-67
Isolation of Rat and Mouse Alveolar Type II Epithelial Cells (Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok et al.)....Pages 69-82
Isolation and Characterization of Human Alveolar Type II Cells (Beata Kosmider, Robert J. Mason, Karim Bahmed)....Pages 83-90
Air–Liquid Interface Culture of Human and Mouse Airway Epithelial Cells (Di Jiang, Niccolette Schaefer, Hong Wei Chu)....Pages 91-109
Isolation and Characterization of Human Lung Myeloid Cells (Yen-Rei A. Yu, Robert M. Tighe)....Pages 111-119
Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells Under Pathological Conditions (Xiaohui Fang, Michael A. Matthay)....Pages 121-128
Measuring Innate Immune Function in Mouse Mononuclear Phagocytes (John Matthew Craig, Neil Raj Aggarwal)....Pages 129-137
Measuring Neutrophil Bactericidal Activity (Kenneth C. Malcolm)....Pages 139-144
Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches (Alexandra L. McCubbrey, William J. Janssen)....Pages 145-168
Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches (Adrianne L. Stefanski, Dorota S. Raclawska, Christopher M. Evans)....Pages 169-201
Computational Analysis of RNA-Seq Data from Airway Epithelial Cells for Studying Lung Disease (Nathan D. Jackson, Lando Ringel, Max A. Seibold)....Pages 203-235
Application of Proteomics in Lung Research (Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, Richard Reisdorph)....Pages 237-262
Application of Metabolomics in Lung Research (Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, Richard Reisdorph)....Pages 263-288
Functional Genomics in Murine Macrophages (Frank Fang-Yao Lee, Scott Alper)....Pages 289-298
Front Matter ....Pages 299-299
Assessment of Ozone-Induced Lung Injury in Mice (Vandy P. Stober, Stavros Garantziotis)....Pages 301-314
Lung Histological Methods (Aneta Gandjeva, Rubin M. Tuder)....Pages 315-329
Intravital Microscopy in the Mouse Lung (Yimu Yang, Joseph A. Hippensteel, Eric P. Schmidt)....Pages 331-339
Mouse Models of Acute Lung Injury and ARDS (Franco R. D’Alessio)....Pages 341-350
Mouse Models of Asthma (Magdalena M. Gorska)....Pages 351-362
Animal Models of Pulmonary Fibrosis (David N. O’Dwyer, Bethany B. Moore)....Pages 363-378
Mouse Models of COPD (Karina A. Serban, Irina Petrache)....Pages 379-394
Mouse Models of Viral Infection (Kerry M. Empey, R. Stokes Peebles Jr, William J. Janssen)....Pages 395-414
Delivery of Therapeutics to the Lung (Dominique N. Price, Pavan Muttil)....Pages 415-429
Back Matter ....Pages 431-433

✦ Subjects

Biomedicine; Molecular Medicine