Luminol chemiluminescence liquid chromatography analysis of rat plasma phosphoglyceride hydroperoxides

✍ Scribed by A.C. Capomacchia; J.-K. Cho; N.H. Do; O.R. Bunce

• Book ID: 102983883
• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 660 KB
• Volume: 266
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85054-a

✦ Synopsis

A novel, normal phase hquld chromatography (LC) system unth chemkumnescence (CL) detectIon was developed to ldentlfy and quantify phosphoglycende hydroperoxldes (PGOOH) m rat plasma Plasma hpld extracts were separated on a 5 pm slhca column (250 mm X 4 6 mm I d ) using methanol-lsopropanol( 91, v/v> A lummol(15 pg ml-') and cytochrome c (15 pg ml-') solution prepared m 0 001 M KOH (pH 11) was used as the CL reagent solution Retention times for the followmg PGOOH were obtamed phosphatldylethanol amme hydroperoxlde (4-5 mm), phosphatldylmosltol hydroperoxlde (6 5-7 5 mm), phosphatidylchohne hydroperoxlde (lo-12 nun) and phosphatldylsenne hydroperoxlde (18-19 mm) LC peaks of the above PGOOH were cahbrated agamst an external standard, teri-butyl hydroperomde The study used female Sprague-Dawley rats admuustered 5 mg kg-' body we&t of dlmethylbenzanthracene (DMBA) mamtamed on a diet wntammg 20% hpld derived from either corn 011, menhaden 011, prnnrose 011 or fish 011 concentrate Phosphatldyhnosltol hydroperoxlde was the only PGOOH detected Its concentration m rat plasma paralleled the rats' tumor burden initiated by DMBA admuustratlon