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Luminescent Terbium Protein Labels for Time-Resolved Microscopy and Screening

✍ Scribed by Harsha E. Rajapakse; D. Rajasekhar Reddy; Shabnam Mohandessi; Nathaniel G. Butlin; Lawrence W. Miller

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 616 KB
Volume: 121
Category: Article
ISSN: 0044-8249
DOI: 10.1002/ange.200900858

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Zum Leuchten gebracht: Heterodimere Konjugate aus Trimethoprim und sensibilisierten Terbiumchelaten koordinieren Fusionsproteine der Dihydrofolat‐Reduktase aus Escherichia coli mit nanomolarer Affinität (siehe Bild). Anhand der Terbiumlumineszenz lassen sich markierte Proteine in vitro und auf der Oberfläche lebender Säugerzellen zeitaufgelöst und mit hoher Empfindlichkeit detektieren.magnified image

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