Luminescence of Ca2+-activated photoprotein obelin initiated by NaOCl and MnCl2

✍ Scribed by Vysotski, Eugene S. ;Trofimov, Konstantin P. ;Bondar', Vladimir S. ;Gitelson, Josef I.

• Book ID: 101717107
• Publisher: John Wiley and Sons
• Year: 1993
• Weight: 354 KB
• Volume: 8
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170080603

✦ Synopsis

The luminescence of obelin is initiated by NaOCl in a reaction mixture containing no calcium. The addition of Mn2+ enhances the light emission >300-fold. Sodium azide and histidine, as singlet oxygen quenchers, inhibit NaOCI-activated obelin luminescence in the presence or absence of Mn". This suggests that the addition of NaOCl to the mixture causes singlet oxygen formation (stimulated by Mn2+ ions), and singlet oxygen initiates the light-emitting reaction.