Luminescence in the study of lipid metabolism

✍ Scribed by Wieland, E. ;Niedmann, D. ;Diedrich, F. ;Seidel, D. ;Kather, H.

• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 730 KB
• Volume: 4
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170040158

✦ Synopsis

Using bioluminescence assays for glycerol, free fatty acids, 0-hydroxybutyrate and lactate, w e were able t o perform complex studies of human energy and lipid metabolism both in serum samples in wiwo and i n isolated fat cells in vitro. These studies would have been impossible without reliable, specific and highly sensitive luminescence methods. Oxidatively modified low density lipoprotein (LDL) has been implicated in the pathogenesis of atherosclerosis. Adaptation of a chemiluminescence assay for lipid hydroperoxides t o LDL isolated by specific precipitation from serum makes it possible t o measure LDL oxidation in wiwo. Cell dependent chemiluminescence was used t o investigate whether receptor mediated endocytosis of LDL by macrophages leads t o oxygen radical production in these cells. No activation of the membrane NADIPIH oxidase was observed.