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Luciferase from the Italian firefly Luciola italica: Molecular cloning and expression

โœ Scribed by Bruce R. Branchini; Tara L. Southworth; Jennifer P. DeAngelis; Aldo Roda; Elisa Michelini

Book ID
116345034
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
738 KB
Volume
145
Category
Article
ISSN
1096-4959

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โœ Tatsumi, Hiroki ;Masuda, Tsutomu ;Kajiyama, Naoki ;Nakano, Eiichi ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons โš– 285 KB

We have cloned the cDNA for luciferase from lantern poly(A)+ RNA of a Japanese firefly, Luciola cruciara (Genji botaru in Japanese). This cDNA directed the synthesis of enzymatically active luciferase under the control of the lac promoter in Escherichia coli. The amino acid sequence predicted from t