Low-temperature glycol methacrylate resin embedding method: A protocol suitable for bone marrow immunohistochemistry, PCR, and fish analysis
✍ Scribed by Qiguo Zhang; Jing Wang; Hongyan Wu; Le Zhang; Jinyong Zhou; Qing Ye; Xiaoyan Shao; Chaoyang Guan; Jingyan Xu; Yonggong Yang; Rongfu Zhou; Jian Ouyang
- Book ID
- 102334866
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 359 KB
- Volume
- 73
- Category
- Article
- ISSN
- 1059-910X
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✦ Synopsis
Abstract
Molecular analyses such as fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) are demanded to improve diagnostic accuracy in addition to immunohistopathology of bone marrow (BM) trephine specimens. Conventional BM embedding method needs decalcification, and its procedure may impair tissue morphology and DNA quality. Here, we report an undecalcified method by which glycol methacrylate resin is polymerized at low temperature (4°C). Using this method, BM enzyme activity and antigenic determinants are well preserved, and moreover, DNA extracted from plastic embedding sections is suitable for PCR amplification and sequencing, FISH analysis can be well done because of the DNA integrity of BM sections. If working with BM trephine specimen, our protocol offers the possibility to combine superior morphology with modern molecular analysis. Microsc. Res. Tech. 73:1067–1071, 2010. © 2010 Wiley‐Liss, Inc.