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Low Oxygen Tension Decreases Receptor Binding of Peptide Growth Factors in Dermal Fibroblast Cultures

✍ Scribed by Vincent Falanga; Hajime Takagi; Patricia I. Ceballos; Jeffrey B. Pardes


Book ID
115601768
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
290 KB
Volume
213
Category
Article
ISSN
0014-4827

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High and low affinity binding sites for
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Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind