✦ LIBER ✦
Low NA+ concentration: A factor contributing to diminished uptake and incorporation of amino acids by diapausing mouse blastocysts?
✍ Scribed by Van Winkle, Lon J.
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 400 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Uptake and incorporation of ^14^C amino acids by normal and diapausing blastocysts was found to be Na^+^ dependent with one exception. Delayed embryos preincubated 25 h in medium containing 124 mM Na^+^ had labeling characteristics independent of the Na^+^ concentration present during labeling. Since diapausing embryos preincubated 25 h in low Na^+^ medium (55 mM) retained their Na^+^ dependent labeling characteristics, delayed blastocysts may be in a low Na^+^ environment in situ. Low Na^+^ may explain why delayed blastocysts are unable to utilize radioactive methionine in vivo (Weitlauf and Greenwald, '68).