Low level expression in Escherichia coli of a fungal gene under the control of strong promoters
✍ Scribed by Christina Patiño; Flora Sánchez; Miguel A. Peñalva
- Book ID
- 109314675
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 458 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0378-1097
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To determine the utility of coupling runaway replication to the expression of cloned genes under the control of strong promoters, lacZ transcriptional fusions to the trp or tac promoter (Ptrp or Ptac) were constructed using plasmids in which the copy number is thermally regulated. Cells containing t