✦ LIBER ✦

Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by Raman and IR spectroscopies

✍ Scribed by M. Picquart; E. Haro-Poniatowski; J. F. Morhange; M. Jouanne; M. Kanehisa

Publisher: Wiley (John Wiley & Sons)
Year: 2000
Tongue: English
Weight: 106 KB
Volume: 53
Category: Article
ISSN: 0006-3525
DOI: 10.1002/(sici)1097-0282(20000405)53:4<342::aid-bip6>3.0.co;2-m

No coin nor oath required. For personal study only.

✦ Synopsis

The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the ␤-sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 and 94 cm Ϫ1 was observed. This band has been attributed to vibrations of elastic body. The results are interpreted using a simple elastic model.