Low dose fractionated radiation enhances the radiosensitization effect of paclitaxel in colorectal tumor cells with mutant p53
✍ Scribed by Damodaran Chendil; Rachael Oakes; Rachael A. Alcock; Nish Patel; Christopher Mayhew; Mohammed Mohiuddin; Vincent S. Gallicchio; Mansoor M. Ahmed
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 217 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
BACKGROUND.
The current study was undertaken to investigate the influence of wild-type or mutant p53 status on the radiosensitizing effect of paclitaxel in colorectal tumor cell lines.
METHODS. HCT-116 (contains wild-type p53) and HT-29 (contains mutant p53) established from moderately differentiated colorectal carcinomas were used in this study. Colony-forming assay was performed after exposure to either different radiation doses (0.5-6 gray [Gy]) or paclitaxel (1-10 nM) or in combination.
Induction of p53 and p21 waf1/cip1 by these treatments were determined by immunocytochemistry and Western blot analysis.
RESULTS.
Radiation caused an increase in nuclear p53 and p21 waf1/cip1 proteins in HCT-116 cells, indicating that p53 functionally induced p21 waf1/cip1 . However, induction of nuclear p53 and p21 waf1/cip1 protein was not evident in HT-29 cells,