Low cost production of perdeuterated biomass using methylotrophic yeasts

✍ Scribed by Stéphane Haon; Sophie Augé; Marielle Tropis; Alain Milon; Nic D. Lindley

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 474 KB
• Volume: 33
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580331108

✦ Synopsis

Abstract

Three strains of methylotrophic yeasts, Candida boidinii, Pichia angusta (previously Hansenula polymorpha) and Pichia Pastoris, were studied for their capacity to grow on methanol in deuterated media. Growth rates, determined relative to the extent of deuteration of water and/or methanol, showed that water deuteration was the major limiting factor. After adaptation to deuterium by progressive transfer through media of increasing deuteration, growth rates were diminished relative to those obtained on hydrogenated media of identical salts composition: the two Pichia species retained the highest growth rates (μ=0.02 h^−1^) in the fully deuterated medium. Perdeuterated biomass (16 g) was obtained in a 1 liter fed‐batch fermentation and the extent of deuteraton of isolated ergosterol has been shown to be 97.5% by mass spectrometric analysis.