Low concentration drug analysis by semi-microcolumn liquid chromatography with a polymer-coated mixed-function precolumn

✍ Scribed by Osamu Shirota; Ayako Suzuki; Taketoshi Kanda; Yutaka Ohtsu; Michihiro Yamaguchi

• Book ID: 102945826
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 546 KB
• Volume: 7
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220070105

✦ Synopsis

Abstract

The construction and performance of dual‐and triple‐column systems for the analysis of dilute drug samples are described. The systems are based on the following two separation processes: (1) deproteinization and analyte fractionation using a short polymer‐coated mixed‐function (PCMF) phase (10 mm × 4.0 mm i.d. column) and, (2) final separation using a C18 semi‐microcolumn (1.5 to 2.0 mm i.d.). The surface structure of the MF phase and the geometry of the packed column for the primary separation made it possible to inject hundreds of microliters of serum samples into the system. Analytes separated in the primary chromatography are transferred to the main column with or without an intermediate column (35 mm × 1.0 mm i.d.). A system using an intermediate column was designed to save time of analysis when a 1.5‐mm i.d.(and smaller) main column was used. The systems showed an increased concentration sensitivity for drug molecules, such as diazepam, carbamazepine, and phenobarbital, without any loss in chromatographic efficiency. Carbamazepine and phenobarbital at 12.5 ppb and 50 ppb, respectively, in human serum were detected with good S/N ratio.