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Loss of Specific Agglutinating Activity of Purified Ferritin-conjugated Antibodies

โœ Scribed by VOGT, ARNOLD; KOPP, RUDOLF


Book ID
109635043
Publisher
Nature Publishing Group
Year
1964
Tongue
English
Weight
371 KB
Volume
202
Category
Article
ISSN
0028-0836

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125I-monoclonal IgG anti-gamma chain antibodies were conjugated to ferritin using glutaraldehyde as a bifunctional reagent. The molar ratio of IgG:ferritin:glutaraldehyde resulting in the highest yield was determined. Free IgG was separated from IgG bound to ferritin by sucrose density gradient ultr