Preparation of monoclonal antibody-ferri
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R. A. Rudick; E. K. Bloechl; D. W. Knutson
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Article
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1984
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Springer
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English
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125I-monoclonal IgG anti-gamma chain antibodies were conjugated to ferritin using glutaraldehyde as a bifunctional reagent. The molar ratio of IgG:ferritin:glutaraldehyde resulting in the highest yield was determined. Free IgG was separated from IgG bound to ferritin by sucrose density gradient ultr