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Loss of membrane proteins from thylakoids during freezing

✍ Scribed by Agnes Mollenhauer; Jürgen M. Schmitt; Sean Coughlan; Ulrich Heber

Book ID
115726388
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
794 KB
Volume
728
Category
Article
ISSN
0005-2736

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Freezing of isolated thylakoid membranes
Freezing of isolated thylakoid membranes in complex media
✍ K. A. Santarius 📂 Article 📅 1986 🏛 Springer-Verlag 🌐 English ⚖ 697 KB

Chloroplast thylakoid membranes isolated from spinach leaves (Spinacia oleracea L. cv. Monatol) were subjected to a freeze-thaw treatment in a buffered medium containing 70 mM KCl, 30 mM NaNO3 and 20 mM K2SO4 in different combinations. In the presence of the three predominant inorganic electrolytes,