Loss of imprinting of igf2 in renal-cell carcinomas

✍ Scribed by Hideaki Oda; Haruki Kume; Yasuhito Shimizu; Tohru Inoue; Takatoshi Ishikawa

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: French
• Weight: 75 KB
• Volume: 75
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19980130)75:3<343::aid-ijc3>3.0.co;2-2

✦ Synopsis

Loss of imprinting (LOI) of the igf 2 and h19 genes has been found not only in embryonal tumors but also in common adult cancers. To determine any possible role of genomic imprinting in the development of renal-cell carcinomas (RCCs), we examined the imprinting status of igf 2 and h19 in a series of 22 such tumors, and studied its relation to their mRNA expression. Of 14 RCC specimens heterozygous for the ApaI polypmorphism, 7 (50%) showed LOI of igf 2. In contrast, for h19 all 9 informative cases maintained imprinting. Furthermore, all 7 cases with LOI transcribed igf 2 mRNA at elevated levels, while H19 expression was low regardless of the imprinting status compared with that of background level in each case. These results suggest that LOI of igf 2, but not of h19, plays a role in the case of human RCC. However, in contrast to that in Wilms' tumor, LOI in RCC was not associated with any specific down-regulation of h19.