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Loss of heterozygosity studies in squamous cell carcinomas of the head and neck

✍ Scribed by David T. Ransom; J. Helen Leonard; John H. Kearsley; Gavin R. Turbett; Kathy Heel; Vilnis Sosars; Nicholas K. Hayward; James F. Bishop

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
800 KB
Volume
18
Category
Article
ISSN
1043-3074

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✦ Synopsis

Background. Loss of heterozygosity (LOH) studies have been pivotal in identifying tumor suppressor genes involved in the pathogenesis of a number of cancers. In squamous cell carcinomas of the head and neck region (SCCHN), LOH studies using the Southern blot technique are scarce.

Methods. SCCHNs were obtained immediately after surgical resection from 78 patients. Histologic confirmation was made by frozen section and tumors with less than 50% malignant cells were excluded. DNA was digested with restriction enzymes, and after Southern blotting the membranes were hybridized with radiolabeled probes. Chromosome arms analyzed included 1 p, 3p, 4p, 5q, 8p, lop, l l p , l l q , 13q. 17p, 17q, 18q, 21q, and22q.

Results. The average rate of LOH was 25% per chromosome arm. Significantly higher rates of LOH were observed for chromosome arms 5q (56%) and 17p (45%). Other investigators have reported high rates of LOH forthe Hras-1 locus, and chromosome arms 11 p, 1 l q , and 13q. However, these results were not confirmed in this study. For patients with stage 1 or 2 tumors, the overall LOH rate was 13%, and for patients with stage 3 or 4

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