Loss of chromosome 3p arm differentiating tumorigenic from non-tumorigenic cells derived from the same SV40-transformed human mammary epithelial cells
✍ Scribed by Jérocme Lebeau; Michèle Gerbault-Seureau; Nicole Lemieux; Francloise Apiou; Fabien Calvo; Philippe Berthon; Gerard Goubin; Bernard Dutrillaux
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 964 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
After immortalization of human normal mammary epithelial cells by replication-defective SV40 genome integration, 2 cultures were developed independently. Both had the same integration site, in band 9q2 I, but rapidly diverged karyotypically. After a few passages, one, designated SCZT2, exhibited neardiploid (a) and the other, designated SL2T2, near-tetraploid @) karyotypes. The simplest formulas were 44, X, -X, der(3;22) (q I0;q lo), der(4) t(4;9)(q34;q I2), +8, +9, add( I3)(p I), der( ) t(& 19)(q2 I ;PI 3 4 , add(22)(p I) for karyotype (a) and 93, XXXX, add( I)(q I2), add( I I)(q 13). + 20 for karyotype (b). A number of alterations were further acquired with passages. Both cell cultures were tumorigenic, but their efficiency of grafting in nude mice largely differed: it was low for SET2 and high for SC2T2 cultures. All cultures of the xenografted tumors, obtained from either SUT2 or SC2T2. exhibited the same clonal anomalies as those characterizing karyotype (a). It was concluded that only cells with karyotype (a) were tumorigenic, and that the difference in the tumorigenic potential of cultures SC2T2 and SUT2 was related to their richness in cells with this karyotype. The comparison of the various karyotypes, together with data obtained in other cell types transformed by SV40, suggests that the acquisition of tumorigenicity in S2T2 mammary epithelial cells may be related to the loss of chromosome 3p arm.