Loofa Sponge as a Scaffold for Culture of Rat Hepatocytes
β Scribed by Jyh-Ping Chen; Tsung-Cheng Lin
- Book ID
- 109387071
- Publisher
- American Institute of Chemical Engineers
- Year
- 2008
- Tongue
- English
- Weight
- 309 KB
- Volume
- 21
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
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## Abstract This study proposes a new threeβdimensional culture of mouse hepatocytes in a porous galactoseβcarrying modified gelatin sponge matrix. The modification of gelatin using galactose residues significantly increased the attachment of hepatocytes on the substrate. A modified gelatin sponge
Adult rat hepatocytes efficiently attach to intact connective tissue fibers prepared from normal rat liver; this material has been given the name of %omatrix". The cells remain alive and differentiated for at least 4 months in culture. The liver biomatrix contains both collagen and noncollagenous gl