Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice
✍ Scribed by Tammy L. Kalber; John C. Waterton; John R. Griffiths; Anderson J. Ryan; Simon P. Robinson
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 400 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1053-1807
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Purpose
To characterize longitudinal tumor progression in a murine orthotopic model of liver metastasis using susceptibility contrast magnetic resonance imaging (MRI).
Materials and Methods
Nude mice were inoculated intrasplenically with LS174T colorectal carcinoma cells 24 hours postadministration of 2.5 mgFe/kg of the ultrasmall superparamagnetic iron oxide particle preparation feruglose. Contiguous T~2~ and T~2~*‐weighted multislice MR images were acquired 10, 15, 20, 25, 30, and 35 days postinoculation to longitudinally evaluate metastatic progression. Functional tumor vasculature and hypoxia were histologically evaluated at the final timepoint using Hoechst 33342 uptake, pimonidazole and hematoxylin and eosin staining. A parallel cohort of subcutaneous tumors was included for comparison.
Results
All intrasplenically inoculated mice developed liver metastases, evident in both T~2~*‐ and T~2~‐weighted images as high‐signal deposits, compared to feruglose‐nulled normal liver. Small lesions were detected as early as day 10 and all mice exhibited progressing lesions over 35 days. Liver metastases took longer to establish, but exhibited a similar volume doubling time to the subcutaneously propagated tumors of ≈2–3 days. Different functional tumor vascular architectures between the two growth sites were apparent.
Conclusion
Susceptibility‐contrast MRI using a single dose of feruglose can be used to easily detect and longitudinally monitor orthotopically propagated liver metastases in vivo. J. Magn. Reson. Imaging 2008;9999:1451–1458. © 2008 Wiley‐Liss, Inc.