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Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544)

✍ Scribed by Anita Neufahrt; F. Jochen Förster; Hartmut Heine; Gertrud Schaeg; Gottfried Leonhardi

Publisher
Springer-Verlag
Year
1976
Tongue
English
Weight
675 KB
Volume
256
Category
Article
ISSN
0340-3696

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Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544)
✍ Anita Neufahrt; Franz Jochen Förster; Karin Gottschalk; Gottfried Leonhardi 📂 Article 📅 1978 🏛 Springer-Verlag 🌐 English ⚖ 233 KB

Human skin epithelial-like cells (NCTC strain 2544) were grown in NCTC 135 medium. Neuraminidase and hyaluronidase were added to the growth medium. Cells were incubated 96 h at 36 degrees C. Growth rate and viscosity of cell suspensions were measured after forming single cells mechanically (mopping)