Long-term survival and plasmid maintenance of Escherichia coli in marine microcosms
β Scribed by Jeffrey J. Byrd; Rita R. Colwell
- Book ID
- 109384638
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 424 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0168-6496
No coin nor oath required. For personal study only.
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The maintenance and stability in an E. coli K12 host of environmental isolates of R plasmids encoding gentamicin resistance during multiple passages in antibiotic-free and antibiotic-containing broth were investigated in regard to the conferred resistance phenotypes and the respective EcoRI digestio
Plasmid R1 was tested for stable maintenance in Escherichia coli K12. Populations carrying a transfer-negative derivative of plasmid R1drd-19 were grown exponentially for 72 generations in LB medium. Out of nearly 5000 cells none had lost the plasmid; hence the rate of loss of the plasmid is less th