Long-term shoot regeneration from pampas grass (Cortaderia selloanaSchult.) through manipulation of growth regulators in vitro
✍ Scribed by Carol Robacker
- Book ID
- 104675145
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 745 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
A regeneration system for 'Pumila' pampas grass (Cortaderia selloana Schult.) that yields plants over many months and allows control of morphogenesis was developed. Immature inflorescence explants cultured for three 4-week passages on MS basal medium supplemented with 4.5 μM 2,4-D and 8.9 μM BA yielded a dark green callus that organized into shoot primordia. Rate of shoot development was increased after transfer of shoot primordia to medium supplemented with 9.8 μM IBA. Subculture every 4-6 weeks onto medium containing IBA yielded a continuous production of shoots. Control of morphogenesis was achieved by transferring cultures to medium containing 4.5 μM 2,4-D and 8.9 μM BA for shoot bud proliferation and to medium containing IBA for shoot production.