Long-term protection against Argentine hemorrhagic fever in Tacaribe virus infected marmosets: Virologic and histopathologic findings
✍ Scribed by Sergio R. Samoilovich; Miguel A. Calello; Rubén P. Laguens; Mercedes C. Weissenbacher
- Book ID
- 102377910
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 478 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
Tacaribe virus may represent a better alternative than attenuated strains of Junin virus (JV) for immunization against Argentine hemorrhagic fever (AHF) because of possible risk of persistent infection or disease associated with five, attenuated strains. Callizhrix jacchus marmosets, which suffer 100% mortality if inoculated with the pathogenic XJ strain of JV, were used to evaluate possible Tacaribe virus persistence, subclinical, or long-term disease and the duration of protection against challenge with JV. Histologic studies did not show pathogenic changes due to Tacaribe virus in primates sacrificed from 7 to 480 days postinoculation (pi). No virus was recovered in tissue samples after primary culture or cocultures with sensitive cells. The presence of anti-Tacaribe neutralizing serum antibodies and protection against pathogenic JV were detected up to 480 days after a singIe dose of Tacaribe virus. However, anti-Junin antibodies were detected only after challenge. In other experiments, protection against JV was evaluated histologically and virologically. Two primates were immunized with Tacaribe virus, challenged with JV, and sacrificed 18 or 21 days later. Subciinicaf histopathologic findings were associated with recovery of JV only by the sensitive primary cdture-coculture techniques. The hmunogenjcity, degree of protection, and safety of Tacaribe virus indicate its potential as a vaccine against human AHF.