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Long-term maintenance of “cloned” human PLT cells in TCGF with LCL cells as a feeder layer

✍ Scribed by Hank, Jacquelyn A. ;Inouye, Hiroo ;Guy, Lynn A. ;Alter, Barbara J. ;Bach, Fritz H.

Publisher: Wiley (John Wiley & Sons)
Year: 1980
Tongue: English
Weight: 415 KB
Volume: 13
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400130411

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✦ Synopsis

Abstract

The long‐term maintenance of T cells “cloned” by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 × 10^12^ cells from a “clone” seeded at one cell per well. Some of the clones tested express primed LD‐typing activity.

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