Long-term induction of c-jun mRNA and jun protein in rabbit retinal ganglion cells following axotomy or colchicine treatment
✍ Scribed by Dr. J. Koistinaho; K. J. Hicks; S. M. Sagar
- Book ID
- 102908508
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 698 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
The expression of the c-jun, c-fos, and NGFI-A genes was studied in the rabbit retina after optic nerve crush (ONC) or an intravitreal injection of colchicine. By Northern blotting, the basal expression of c-fos and NGFI-A mRNAs were undetectable, whereas cjun mRNA showed a low basal expression in shamoperated control retinas. Very few or no Jun-or Fosimmunoreactive nuclei were seen in control retinas. From 1 to 95 days after ONC a marked induction of JUN-but not FOS-immunoreactive neurons was seen in the ganglion cell layer peaking at 3 and 7 days.
Jun-positive neurons also accumulated immunoreactive phosphorylated neurofilaments, indicating that they were ganglion cells. Northern blots demonstrated that retinal levels of c-jun mRNA, but not of c-fos or NGFI-A mRNAs, were increased 3 and 7 days after ONC. An intravitreal injection of colchicine also induced Jun-immunoreactivity within 24 hr in most of the neurons in the ganglion cell layer, but not in the inner nuclear and outer nuclear layers. The results indicate that axonal damage induces a specific pattern of IEG expression including a long-term induction of the c-jun gene in CNS neurons.