Long-term effectiveness of photodynamic therapy by using a hydrophilic photosensitizer ATX-S10(Na) against experimental choroidal neovascularization in rats
✍ Scribed by Kanai, Masakazu; Obana, Akira; Gohto, Yuko; Nagata, Satoshi; Miki, Tokuhiko; Kaneda, Kenji; Nakajima, Susumu
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 694 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and objective:
We previously demonstrated that a hydrophilic photosensitizer atx-s10 had a potent photodynamic effect. this study was designed to reveal the long-term effectiveness of photodynamic therapy (pdt) with this agent in occluding choroidal neovascularization (cnv) and its selectivity in the neovascular tissue.
Study design/materials and methods:
Experimental cnv was induced by intense photocoagulation in rat eyes. immediately or 2 hours after intravenous injection of 8 mg/kg body weight of atx-s10(na), a cis isomer of atx-s10, eyes were irradiated by a diode laser at the radiance of 3.25-65.3 j/cm(2) vascular occlusion was identified by fundus photography, fluorescein angiography, and histology at 1, 3, 7, 14, and 28 days after pdt. as controls, non-neovascular eyes were subjected to pdt and similarly analyzed.
Results:
By using the following treatment parameters, pdt with atx-s10(na) successfully occluded cnv without causing occlusion of retinal capillaries for 28 days; 7.4 and 19.6 j/cm(2) immediately after dye injection and 36.7 and 65.3 j/cm(2) 2 hours after injection. although these conditions also caused occlusion of normal choriocapillaries and mild injuries of retinal vessels, retinal pigment epithelium, and photoreceptors at 1 day, retinal vessels and pigment epithelial cells recovered from damages by 28 days. no injuries were found in the inner retina.
Conclusion:
In optimal treatment conditions, pdt with atx-s10(na) can induce long-term, selective occlusion of cnv without causing irreversible damages in the inner retina.