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Long-term culture of primary hepatocytes: new matrices and microfluidic devices

✍ Scribed by Burkhardt, Britta; Martinez-Sanchez, Juan José; Bachmann, Anastasia; Ladurner, Ruth; Nüssler, Andreas K.

Book ID
121567098
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
286 KB
Volume
8
Category
Article
ISSN
1936-0533

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Rat hepatocytes were seeded on three-dimensional highly porous polyvinylalcohol (PVA) and aminoethyl-modified polyvinylalcohol (AE-PVA) matrices. Hepatocytes were cultured under static and dynamic conditions. The three-dimensional matrices offered an improved extracellular microenvironment for long-