Transfer of foreign gene to giant freshw
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Si-Shen Li; Huai-Jen Tsai
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Article
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2000
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John Wiley and Sons
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English
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We developed a spermatophoremicroinjection (SMI) technique that allows exogenous DNA fragments to be transferred easily into the giant freshwater prawn (Macrobrachium rosenbergii), an important aquacultural shellfish and aquatic invertebrate model. From 28 to 1,000 ng of the circular plasmid pGL, in