Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 ofB. patellarisandB. procumbens
✍ Scribed by E. M. J. Salentijn; N. N. Sandal; R. Klein-Lankhorst; W. Lange; Th. S. M. Bock; K. A. Marcker; W. J. Stiekema
- Book ID
- 104671675
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 854 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0040-5752
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✦ Synopsis
New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 (pat-1) locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 (pro-1) locus.