Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae

✍ Scribed by Lawther, Robert P. ;Phillips, Stephen L. ;Cooper, Terrance G.

• Book ID: 104730747
• Publisher: Springer
• Year: 1975
• Tongue: English
• Weight: 645 KB
• Volume: 137
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00341675

✦ Synopsis

The RNA polymerase inhibitor, lomofungin has been used to determine the half life of specific synthetic capacities (invertase and alpha-glucosidase) as well as that for gross protein synthesis. In both cases the studies conclude that cognate messenger RNAs decay with a half life of approximately 20 minutes. This antibiotic has been used to determine the half life of allophanate hydrolase specific synthetic capacity. We find that it decays with a half life of about three minutes; a value that agrees with the decay rates of allophanate hydrolase synthetic capacity following removal of inducer. These observations argue that mRNA may be metabolized by two separate routes in Saccharomyces.