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Locking the DNA Gate of DNA Gyrase: Investigating the Effects on DNA Cleavage and ATP Hydrolysis †

✍ Scribed by Williams, Nicola L.; Maxwell, Anthony

Book ID
127083629
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
288 KB
Volume
38
Category
Article
ISSN
0006-2960

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This study deals with the effects of a temperature-sensitive (ts) mutation at the gene encoding the DNA gyrase B subunit (gyrBts) and a deletion of the top gene encoding the omega protein upon the superhelical density of the pAO3 plasmid in E. coli cells. The alteration of the DNA gyrase B subunit i