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Location of the origin-terminus of the viral strand of the duplex replicative form of bacteriophage G4 DNA

โœ Scribed by Dan S. Ray; Jeanene Dueber

Book ID
118917938
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
711 KB
Volume
113
Category
Article
ISSN
0022-2836

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A general method has been developed for the large scale isolation of intact, circular, single-stranded DNA molecules of each strand from supercoiled duplex DNA. The method involves the conversion of the supercoiled duplex DNA to singly nicked, relaxed duplex DNA; denaturation of the duplex DNA; sepa