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Location of the disulfide bonds in the antitumor protein neocarzinostatin

✍ Scribed by Kenji Kuromizu; Okitoshi Abe; Hiroshi Maeda


Book ID
115708706
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
519 KB
Volume
286
Category
Article
ISSN
0003-9861

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The thermostable sweet protein brazzein consists of 54 amino acid residues and has four intrumolecular disulfide bonds, the location qf which is unknown. We found that bruzzein resists enzymatic hydrol-vsis at enzyme/substrute ratios ( w / w ) of 1:100-1:10 at 35-40Β°C for 24-48 h. Brazzein wus hjdro