Location of terminal methyl groups of acyl chains in the bilayer of unilamellar phosphatidylcholine liposomes: a 13C-NMR study

✍ Scribed by D. Uhríková; P. Balgavý

• Book ID: 103039521
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 368 KB
• Volume: 47
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(88)90034-5

✦ Synopsis

The spin-lattice ~3C-NMR relaxation time 7"1 of carbons in egg yolk phosphatidylcholine (EYPC) unilamellar liposomes was measured at 15 MHz, 25 MHz and 75 MHz in the presence of diamagnetic La 3÷ and paramagnetic Gd ~÷ ions. Supposing formation of ML 2 complexes (where M is the metal ion and L the lipid) and using a simplified Solomon--Bloembergen--Morgan equation, a value of 2 = 0.1880 ± 0.0005 nm -6 was obtained for C carbon of lipid chains, where ris is the distance of Gd 3÷ unpaired electron and C nucleus, and angle brackets denote a weighted average. This value may serve as the basis for testing the application of statistical mechanics to lipid chain conformation and chain terminal group distribution in lipid bilayers.