Location and interaction of N-(9-anthroyloxy)-stearic acid probes incorporated in phosphatidylcholine vesicles

✍ Scribed by José Villalaín; Manuel Prieto

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 486 KB
• Volume: 59
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(91)90058-j

✦ Synopsis

The set of n-(9-anthroyloxy)-stearic acid (n-AS) probes (n = 2, 3. 6, 9, 12) was studied in small unilamellar vesicles of dipalmitoylphosphatidylcholine by nuclear magnetic resonance spectroscopy (anisotropic diamagnetic effects and relaxation experiments) and in multilamellar vesicles by differential scanning calorimetry. From our results it can be concluded that (i) the probes are located at a graded series of depths, (ii) the induced perturbations on the membrane are of long range, (iii) 2-AS is adsorbed on the membrane interface and (iv) incorporation of 9-AS induces the greatest membrane perturbation.