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Localization of the renal Na−K−Cl cotransporter gene (Slc 12a1) on mouse Chromosome 2

✍ Scribed by S. E. Quaggin; J. A. Payne; B. Forbush; P. Igarashi

Publisher: Springer-Verlag
Year: 1995
Tongue: English
Weight: 234 KB
Volume: 6
Category: Article
ISSN: 0938-8990
DOI: 10.1007/bf00356178

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✦ Synopsis

This order was 506 times more likely than the next most likely order. Method o f mapping: The Jackson Laboratory interspecific backcross panel BSB was used. The panel consisted of 94 N e progeny derived from matings of (C57BL/6J x M. spretus)F~ hybrid females and C57BL/6J males [1]. Database deposition information: MGD accession number MGD-CREX-318. Molecular reagents: A cDNA corresponding to nucleotides 1-471 of the murine Slc12a1 sequence (GenBank accession number U20975) was amplified from mouse kidney poly(A) + RNA by ligation-anchored PCR. The cDNA was cloned into pCRII (Invit-

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