Localization of the 8;13 translocation breakpoint associated with myeloproliferative disease to a 1.5 mbp region of chromosome 13

✍ Scribed by Helena Kempski; Donald Macdonald; Antony J. Michalski; Terry Roberts; John M. Goldman; Nicholas C. P. Cross; Dr.John K. Cowell

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 532 KB
• Volume: 12
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870120408

✦ Synopsis

There are five reported cases of an atypical myeloproliferative disorder in which the leukemia cells have a consistent t(8; I 3)(p I I ;q 12) tiranslocation. We analyzed the breakpoint in metaphases from two of these patients by fluorescence in situ hybridization using a series of yeast artificial chromosomes (YACs) derived from the 13q I 2 region. We found that a YAC containing the FLTl and FLT3 oncogenes was localized distal to the 13q I 2 breakpoint and was not rearranged. YAC 66, a YAC that lies immediatc4y adjacent to the chromosome I 3 centromere, was localized proximal t o the I 3q I 2 breakpoint and was not rearranged. A third YAC, which is located between FLTl and YAC 66, was unrearranged in normal metaphase chromosomes, but showed hybridization signals on both derivative chromosomes in both cases. Thus, the breakpoints in these two cases are localized t o the sarne I .5 Mbp region of 13q 12. This may be the site of an unidentified gene involved in the pathogenesis of some types of leukemia. Genes Chrornosorn Cancer 12:283-287 (1995).