Localization of Swallow-Green fluorescent protein in Drosophila oogenesis and implications for the role of Swallow in RNA localization

✍ Scribed by Edwin C. Stephenson

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 262 KB
• Volume: 39
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/gene.20057

✦ Synopsis

Abstract

The localization of a hybrid protein composed of swallow and Green Fluorescent Protein (GFP) during Drosophila oogenesis is reported. I constructed a hybrid gene with GFP inserted into an internal position of swallow. This gene was integrated into the Drosophila genome and provides full swallow^+^ function, as assayed by the complete rescue of strong swallow mutants. Swallow‐GFP is localized at all points along the oocyte cortex from vitellogenic stages of oogenesis through the end of oogenesis. Higher concentrations of swallow‐GFP are present at the anterior oocyte cortex than at the lateral and posterior oocyte cortices at Stages 10 and 11, when bicoid and htsN4 mRNA transport from nurse cells and localization in the oocyte are most active. At Stage 9 and at Stages 12–14 swallow‐GFP is equally distributed at the anterior, lateral, and posterior oocyte cortices. The position of swallow‐GFP in vitellogenic stages is identical to the position of endogenous swallow protein determined by indirect immunofluorescence using an anti‐swallow antibody. At the oocyte cortex, swallow‐GFP is present in particulate structures that lie within or just internal to the dense cortical actin meshwork. These particles show little or no movement, suggesting that they are attached to or embedded in the oocyte cortex. These observations are most easily interpreted in the context of mRNA anchoring or microtubule organizing functions for the swallow protein. genesis 39:280–287, 2004. © 2004 Wiley‐Liss, Inc.