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Localization of spindle checkpoint proteins in cells undergoing mitosis with unreplicated genomes

โœ Scribed by Johnson, Mary Kathrine ;Cooksey, Amanda M. ;Wise, Dwayne A.

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
315 KB
Volume
65
Category
Article
ISSN
0886-1544

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Chinese hamster ovary (CHO) cells, which are arrested at the G1/S-phase of the cell cycle with hydroxyurea, enter mitosis prematurely when treated with caffeine [Schlegel and Pardee, 1986; Science 233-1264-1266]. Such mitotic cells with unreplicated genomes (MUGs) can assemble a mitotic spindle and