Localization of rhinovirus replication in vitro with in situ hybridization

✍ Scribed by Dr. Eurico de Arruda III; Theodore E. Mifflin; Jack M. Gwaltney Jr.; Birgit Winther; Frederick G. Hayden

• Book ID: 102909026
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 904 KB
• Volume: 34
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890340107

✦ Synopsis

To facilitate understanding of human rhinovirus (HRV) pathogenesis, methods were developed for detection of HRV infection in vitro using in situ hybridization (ISH). HRV-14 RNA probes and oligonucleotide probes representing conserved sequences in the 5'-non-translated region were labeled with 35S and used to detect infected HeLa or WI-38 strain human embryonic lung cells in cytological preparations. ISH was shown to be specific for detection of HRV on a single-cell basis. Subsequently, in human nasal polyps infected in vitro, both oligonucleotide- and ribo-probes produced a strong signal in association with ciliated epithelial cells. In human adenoids infected in vitro, a signal was observed in non-ciliated epithelial cells. This study shows that HRV replicates in ciliated cells in the epithelium of human nasal polyps infected in vitro, and the presence of viral RNA in non-ciliated cells of the human adenoid infected in vitro suggests that other cell types may also support rhinovirus replication.