Karyotype analyses based on staining by acetocarmine followed by Giemsa N-banding of somatic metaphase chromosomes of Hordeum vulgare L. were carried out on 61 reciprocal translocations induced by X-irradiation. By means of computer-based karyotype analyses all of the 122 breakpoints could be locali
Localization of NORs in spermatogonial metaphase chromosomes of six species of grasshoppers
β Scribed by J. S. Yaday; A. S. Yaday
- Publisher
- Springer Netherlands
- Year
- 1987
- Tongue
- English
- Weight
- 828 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0016-6707
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β¦ Synopsis
The silver staining technique was employed to locate Nucleolar Organiser Regions (NORs) in six species of grasshoppers viz. Aiolopus thalassinus E (Tryxalinae); Oeodaleus abruptus Thunb., Gastrimargus transversus Thunb., Heteropternis respondens Walk. (Oedipodinae); Parahieroglyphus biliniatus Bol. and Spathosternum prasiniferum Walk. (Catantopinae). Usually the NORs were located on the larger elements of the chromosomal complement. However, in O. abruptus NORs were found on autosomes $8 and $9. The salient observations were: (1) NORs were seen in only a few of the several spermatogonial metaphases examined; (2) Active NORs were mostly located either on one chromatid of the homologues or on the homologue depicting heteromorphism; (3) NORs showed either proximal, subproximal or interstitial locations. However, in O. abruptus and P bilineatus NORs were located at two positions. Distribution of NORs in different species and their probable role in tracing the evolutionary pathways in Acridoidea are discussed.
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