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Localization of MRNA of procollagen type I in supraspinatus tendons of incomplete-thickness rotator cuff tears by in situ hybridization

✍ Scribed by K. Hamada; H. Fukuda; A. Tomonaga; M. Gotoh


Book ID
117715520
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
114 KB
Volume
4
Category
Article
ISSN
1532-6500

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## Abstract To determine the healing potential and healing process of torn supraspinatus tendons, __in situ__ hybridization was used to localize cells containing Ξ±1 type‐I procollagen mRNA. Biopsy specimens of torn supraspinatus tendons from 19 patients with complete‐thickness tears and 13 patients

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Messenger RNAs (mRNAs) encoding procollagen cd type I, cd type II and cd type III have been localized in paraffin sections of human fetal fingers using digoxigenin-labelled synthetic oligonucleotide probes. The probe-mRNA hybrids were visualized using an anti-digoxin antibody amplified with sandwich