Localization of MPM-2 recognized phosphoproteins and tubulin during cell cycle progression in synchronized Vicia faba root meristem cells.
✍ Scribed by P. Binarova; J. Cihalikova; J. Dolezel
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 837 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1065-6995
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
MPM‐2 antibody reacts with a subset of mitotic phosphoproteins. We followed localization of MPM‐2 immunoreactive material and localization of microtubules during cell cycle progression in a highly synchronous population of Vicia faba root meristem cells and isolated nuclei. The MPM‐2 antibody labelling showed significant cell cycle dependence. MPM‐2 nuclear reactivity was weak and homogeneous in G1 and S phase of the cell cycle and became stronger and heterogeneous during G2, resembling staining of the nuclear matrix, with maximum staining at the G2/M interface. Similarly the staining intensity of nucleoli increased from late G1 phase to nucleoli dispersion in early prophase. During mitosis MPM‐2 immunoreactivity was associated with spindle configurations and the brightest signal was localized in kinetochores from prophase to metaphase.